lps-eb vaccigrade Search Results


lipopolysaccharide  (InvivoGen)
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InvivoGen lipopolysaccharide
a, Schematic illustration showing the working principle of immune response induced by <t>LPS</t> administration, cytokine generation and administration of the microneedle patch for longitudinal detection. b, Timeline of endotoxic shock (LPS injection), longitudinal microneedle administration on mice, and blood collection (for validation of the microneedle test). c, Fluorescence map representing mouse IL-6 captured on the microneedles before and at 1, 2.5 and 4 hours post LPS injection. Scale bar, 200 μm. d, Plot depicting concentrations of mouse IL-6 in ISF before and at 1, 2.5 and 4 hours post LPS/saline injection measured using microneedle. (n=3 in each group) Data statistically significant, **** P< 0.0001 by two-way analysis of variance (ANOVA) with Sidak’s multiple-comparison test. e, Plots showing the comparison of concentrations of IL-6 measured in serum and ISF in saline group (left) and LPS group (right). (n=3 in each group) At 4 hours post LPS injection, IL-6 in dermal ISF determined by plasmonic-fluor enhanced microneedle (blue) exhibited good qualitative correlation with that in serum tested by conventional ELISA (grey). Error bars represent standard deviation. Error bars of serum results are technical repeats.
Lipopolysaccharide, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lps-eb+vaccigrade/LPS-EB+VacciGrade/pmc08020465-529-21-22
Average 93 stars, based on 1 article reviews
lipopolysaccharide - by Bioz Stars, 2026-10
93/100 stars
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a, Schematic illustration showing the working principle of immune response induced by LPS administration, cytokine generation and administration of the microneedle patch for longitudinal detection. b, Timeline of endotoxic shock (LPS injection), longitudinal microneedle administration on mice, and blood collection (for validation of the microneedle test). c, Fluorescence map representing mouse IL-6 captured on the microneedles before and at 1, 2.5 and 4 hours post LPS injection. Scale bar, 200 μm. d, Plot depicting concentrations of mouse IL-6 in ISF before and at 1, 2.5 and 4 hours post LPS/saline injection measured using microneedle. (n=3 in each group) Data statistically significant, **** P< 0.0001 by two-way analysis of variance (ANOVA) with Sidak’s multiple-comparison test. e, Plots showing the comparison of concentrations of IL-6 measured in serum and ISF in saline group (left) and LPS group (right). (n=3 in each group) At 4 hours post LPS injection, IL-6 in dermal ISF determined by plasmonic-fluor enhanced microneedle (blue) exhibited good qualitative correlation with that in serum tested by conventional ELISA (grey). Error bars represent standard deviation. Error bars of serum results are technical repeats.

Journal: Nature biomedical engineering

Article Title: Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid

doi: 10.1038/s41551-020-00672-y

Figure Lengend Snippet: a, Schematic illustration showing the working principle of immune response induced by LPS administration, cytokine generation and administration of the microneedle patch for longitudinal detection. b, Timeline of endotoxic shock (LPS injection), longitudinal microneedle administration on mice, and blood collection (for validation of the microneedle test). c, Fluorescence map representing mouse IL-6 captured on the microneedles before and at 1, 2.5 and 4 hours post LPS injection. Scale bar, 200 μm. d, Plot depicting concentrations of mouse IL-6 in ISF before and at 1, 2.5 and 4 hours post LPS/saline injection measured using microneedle. (n=3 in each group) Data statistically significant, **** P< 0.0001 by two-way analysis of variance (ANOVA) with Sidak’s multiple-comparison test. e, Plots showing the comparison of concentrations of IL-6 measured in serum and ISF in saline group (left) and LPS group (right). (n=3 in each group) At 4 hours post LPS injection, IL-6 in dermal ISF determined by plasmonic-fluor enhanced microneedle (blue) exhibited good qualitative correlation with that in serum tested by conventional ELISA (grey). Error bars represent standard deviation. Error bars of serum results are technical repeats.

Article Snippet: For each mouse in vaccination group, cocaine vaccination solution including 100 μg cocaine-BSA conjugate (Fitzgerald Industries, PART# 80–1037) and 2 μg lipopolysaccharide (InvivoGen, catalog code: vac-3pelps) in 100 μl saline mixed with 100 μl 2% Alhydrogel (InvivoGen, catalog code: vac-alu-250) was freshly prepared prior to vaccination.

Techniques: Injection, Biomarker Discovery, Fluorescence, Saline, Comparison, Enzyme-linked Immunosorbent Assay, Standard Deviation